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Nursing: Universal Protocol and Skills Update, Medicine, Page 167

Nursing: Universal Protocol and Skills Update

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Pediatric / Special Population Standard
Procedure for Blood Culture Collection
7. Prepare the site for venipuncture. This is
accomplished by scrubbing the site for a minimum
of 60 seconds with an approved chlorhexidine
gluconate swab in patients over two months of
age. A 70% isopropyl alcohol pad should be utilized
with patients of age two months or less. A circular
area of approximately 3 cm in all directions from
the intended puncture site should be disinfected.
Allow the site to air-dry without assistance such as
fanning. Dry times will vary according to
manufacturer specifications, but typically,
anywhere from 30 seconds to two minutes is
recommended.