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Nursing: Universal Protocol and Skills Update, Medicine, Page 157

Nursing: Universal Protocol and Skills Update

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Adult Modified Procedure for Blood Culture
Collection Involving an Existing Central Line
8. Draw from the specified site / port. Sterilize the port to
be used with a 70% isopropyl alcohol pad and allow
for a minimum of 30 seconds drying time without
assistance such as fanning. Take care when drawing
from the site so as not to contaminate the tip of the
syringe used to inoculate the culture bottles. Inoculate
the aerobic bottle first, then the aerobic bottle, being
careful not to introduce air into the second bottle.
Only fill the bottles to the 10 mL mark, do not overfill.
Other lab draws should be deferred to the
venipuncture site if ordered.